Solution Information | help | |
Enzyme: | Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 | |
inhibitor: | BDBM34130 | |
substrate: | BDBM2 | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.3 | |
Temp. Prep.: | 37 | |
Comments: | n/a | |
Solute 1: | pyruvate kinase/ lactate dehydrogenase | |
Concentration: | 12 / 8.4 U/mL | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | CaCl2 | |
Concentration: | 0.7 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | NADH | |
Concentration: | 0.4 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | phosphoenolpyruvate | |
Concentration: | 1.5 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | 2- amino-2-(hydroxymethyl)-1,3-propanediol | |
Concentration: | 20 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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